Genetic variation of local fish populations after habitat fragmentation: A case study of river at Amphoe Bo kluea, Nan Province
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The objective of this study was to evaluate the effect of barrier on genetic diversity and genetic differentiation of local fish. The local fish, Garra cambodgiensis were collected from 3 locations: Sapun waterfall (SPU/SPL), Sawanua dam (SWU/SWL) and Nakham dam (NKU/NKL). The microsatellite 5 loci were used. The lowest genetic diversity were found in Sapun waterfall, the average allele per locus 7.20 (SPU) - 7.60 (SPL), Effective number of allele 3.07 (SPU) - 5.132 (SPL), Allelic richness 6.986 (SPU) - 7.600 (SPL), observed heterozygosity ( Ho) 0.543 (SPU) - 0.593 (SPL) and expected heterozygosity, (He) 0.647 (SPU) - 0.798 (SPL). The Sawanua dam, allele per locus 7.80 (SWU,SWL), effective number of allele 4.739 (SWU) - 4.057 (SWL), allelic richness 7.800 (SWU, SWL), Ho 0.684 (SWL) - 0.723 (SWU), He 0.716 (SWL) - 0.766 (SWU) and the Nakham dam, allele per locus 8.20 (NKL) – 8.80 (NKU), effective number of allele 5.003 (NKL) – 5.607 (NKU), allelic richness 8.200 (NKL) – 8.663 (NKU), Ho 0.724 (NKU) – 0.748 (NKL) and He 0.776 (NKL) – 0.806 (NKU). Almost samples were in Hardy–Weinberg equilibrium (HWE), except SPL. The genetic differentiation between upper and lower was found in 3 locations, the genetic distance = 0.238, 0.199 and 0.171 and the FST = 0.068, 0.030 and 0.037 in Sapun waterfall, Nakham dam and Sawanua dam consequently. In summary, our results suggest that the barrier was effected on genetic diversity and genetic differentiation of local fish.
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